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  文件名称:  全自动圆色CD光谱仪自动检测蛋白质溶液在远紫外区的圆色(CD) 信号
  公司名称:  英国应用光物理公司上海代表处
  下载次数:   708
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Automated measurement of protein conformation
 
David Gregson
Managing Director
Applied Photophysics Ltd.
 
A stable, native conformation is essential to the correct functioning of a protein. Failure to fold into the correct conformation will cause the protein to exhibit different properties, making it at best inactive or, worse, toxic or immunogenic. Circular dichroism (CD) spectroscopy is a sensitive probe of protein conformation but is not used more routinely because of the perception that it is a labour intensive, low-productivity technique whose results require expert interpretation. In this talk, we shall show how CD can be used early in the development cycle to give additional insights into the likelihood of future success for bio-therapeutic candidates. We will describe a system that:
 
  • sequentially delivers samples to the spectrometer cuvette from a microtitre plate with effectively zero inter-sample carry-over
  • automatically acquires and stores spectroscopic data for up to 384 (4 x 96-well plate) samples without operator intervention
  • recovers sample to minimize waste
  • simultaneously measures more than one optical property (e.g. CD and absorption) to maximize the information return per sample
  • interfaces seamlessly with data reduction programs designed to simplify the interpretation of spectroscopic data
 
Examples using model proteins and those typical in bio-therapeutic development will be presented.
 
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