Automated measurement of protein conformation
Applied Photophysics Ltd.
A stable, native conformation is essential to the correct functioning of a protein. Failure to fold into the correct conformation will cause the protein to exhibit different properties, making it at best inactive or, worse, toxic or immunogenic. Circular dichroism (CD) spectroscopy is a sensitive probe of protein conformation but is not used more routinely because of the perception that it is a labour intensive, low-productivity technique whose results require expert interpretation. In this talk, we shall show how CD can be used early in the development cycle to give additional insights into the likelihood of future success for bio-therapeutic candidates. We will describe a system that:
Examples using model proteins and those typical in bio-therapeutic development will be presented.